Microautoradiography of water-soluble compounds in plant tissue after freeze-drying and pressure infiltration with epoxy resin.
Identifieur interne : 004E33 ( Main/Exploration ); précédent : 004E32; suivant : 004E34Microautoradiography of water-soluble compounds in plant tissue after freeze-drying and pressure infiltration with epoxy resin.
Auteurs : T C Vogelmann [États-Unis] ; R E DicksonSource :
- Plant physiology [ 0032-0889 ] ; 1982.
Abstract
It is difficult to retain and localize radioactive, water-soluble compounds within plant cells. Existing techniques retain water-soluble compounds with varying rates of efficiency and are limited to processing only a few samples at one time. We developed a modified pressure infiltration technique for the preparation of microautoradiographs of (14)C-labeled, water-soluble compounds in plant tissue. Samples from cottonwood (Populus deltoides Bartr. ex Marsh.) labeled with (14)C were excised, quick frozen in liquid N(2), freeze-dried at -50 degrees C, and pressure-infiltrated with epoxy resin without intermediate solvents or prolonged incubation times. The technique facilitates the mass processing of samples for microautoradiography, gives good cellular retention of labeled water-soluble compounds, and is highly reproducible.
DOI: 10.1104/pp.70.2.606
PubMed: 16662542
PubMed Central: PMC1067196
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microautoradiography of water-soluble compounds in plant tissue after freeze-drying and pressure infiltration with epoxy resin.</title><author><name sortKey="Vogelmann, T C" sort="Vogelmann, T C" uniqKey="Vogelmann T" first="T C" last="Vogelmann">T C Vogelmann</name><affiliation wicri:level="2"><nlm:affiliation>United States Department of Agriculture, Rhinelander, Wisconsin 54501.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>United States Department of Agriculture, Rhinelander</wicri:cityArea></affiliation></author><author><name sortKey="Dickson, R E" sort="Dickson, R E" uniqKey="Dickson R" first="R E" last="Dickson">R E Dickson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1982">1982</date><idno type="RBID">pubmed:16662542</idno><idno type="pmid">16662542</idno><idno type="pmc">PMC1067196</idno><idno type="doi">10.1104/pp.70.2.606</idno><idno type="wicri:Area/Main/Corpus">004E33</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004E33</idno><idno type="wicri:Area/Main/Curation">004E33</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004E33</idno><idno type="wicri:Area/Main/Exploration">004E33</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microautoradiography of water-soluble compounds in plant tissue after freeze-drying and pressure infiltration with epoxy resin.</title><author><name sortKey="Vogelmann, T C" sort="Vogelmann, T C" uniqKey="Vogelmann T" first="T C" last="Vogelmann">T C Vogelmann</name><affiliation wicri:level="2"><nlm:affiliation>United States Department of Agriculture, Rhinelander, Wisconsin 54501.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Wisconsin</region></placeName><wicri:cityArea>United States Department of Agriculture, Rhinelander</wicri:cityArea></affiliation></author><author><name sortKey="Dickson, R E" sort="Dickson, R E" uniqKey="Dickson R" first="R E" last="Dickson">R E Dickson</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="1982" type="published">1982</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It is difficult to retain and localize radioactive, water-soluble compounds within plant cells. Existing techniques retain water-soluble compounds with varying rates of efficiency and are limited to processing only a few samples at one time. We developed a modified pressure infiltration technique for the preparation of microautoradiographs of (14)C-labeled, water-soluble compounds in plant tissue. Samples from cottonwood (Populus deltoides Bartr. ex Marsh.) labeled with (14)C were excised, quick frozen in liquid N(2), freeze-dried at -50 degrees C, and pressure-infiltrated with epoxy resin without intermediate solvents or prolonged incubation times. The technique facilitates the mass processing of samples for microautoradiography, gives good cellular retention of labeled water-soluble compounds, and is highly reproducible.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">16662542</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>2</Issue><PubDate><Year>1982</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Microautoradiography of water-soluble compounds in plant tissue after freeze-drying and pressure infiltration with epoxy resin.</ArticleTitle><Pagination><MedlinePgn>606-9</MedlinePgn></Pagination><Abstract><AbstractText>It is difficult to retain and localize radioactive, water-soluble compounds within plant cells. Existing techniques retain water-soluble compounds with varying rates of efficiency and are limited to processing only a few samples at one time. We developed a modified pressure infiltration technique for the preparation of microautoradiographs of (14)C-labeled, water-soluble compounds in plant tissue. Samples from cottonwood (Populus deltoides Bartr. ex Marsh.) labeled with (14)C were excised, quick frozen in liquid N(2), freeze-dried at -50 degrees C, and pressure-infiltrated with epoxy resin without intermediate solvents or prolonged incubation times. The technique facilitates the mass processing of samples for microautoradiography, gives good cellular retention of labeled water-soluble compounds, and is highly reproducible.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vogelmann</LastName><ForeName>T C</ForeName><Initials>TC</Initials><AffiliationInfo><Affiliation>United States Department of Agriculture, Rhinelander, Wisconsin 54501.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dickson</LastName><ForeName>R E</ForeName><Initials>RE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1982</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1982</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1982</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16662542</ArticleId><ArticleId IdType="pmc">PMC1067196</ArticleId><ArticleId IdType="doi">10.1104/pp.70.2.606</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Ultrastruct Res. 1969 Jan;26(1):31-43</Citation><ArticleIdList><ArticleId IdType="pubmed">4887011</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1972 Feb;49(2):161-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16657916</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1972 Feb;49(2):166-71</Citation><ArticleIdList><ArticleId IdType="pubmed">16657917</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1975 Aug;56(2):185-93</Citation><ArticleIdList><ArticleId IdType="pubmed">16659271</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Wisconsin</li></region></list><tree><noCountry><name sortKey="Dickson, R E" sort="Dickson, R E" uniqKey="Dickson R" first="R E" last="Dickson">R E Dickson</name></noCountry><country name="États-Unis"><region name="Wisconsin"><name sortKey="Vogelmann, T C" sort="Vogelmann, T C" uniqKey="Vogelmann T" first="T C" last="Vogelmann">T C Vogelmann</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004E33 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004E33 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:16662542
|texte= Microautoradiography of water-soluble compounds in plant tissue after freeze-drying and pressure infiltration with epoxy resin.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16662542" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |